Nocebo testing and CPT were performed in both groups 30 minutes before this procedure on days 2 and 3, respectively. The Somatosensory Amplification Scale (SSAS Illness Behaviour Questionnaire, and Somatoform Dissociation Questionnaire 20 were used to investigate somatoform dimensions. All participants included in this study were female (aged 2045 years) with a regular menstrual cycle. No participants were taking any drug treatments for disease (including pain syndromes or the contraceptive pill. Subjects were excluded if they had any organic disease (diabetes, cardiac disease, hormonal dysfunction, or hepatic or renal disease). A structured interview was used to collect demographic and familial data, life events, daily and social activities, as well as their lifetime medical conditions.
A blood sample was then taken between 10:00 and 10:30 am, roughly 30 minutes after the pain stimulus was administered. All sessions were considered as stressful, as even the first baseline measurement involved blood being taken, likely to cause a certain degree of stress in the unaccustomed participant.